Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband™ (monoclonal, 2C7G1)

Product Name

Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband™ (monoclonal, 2C7G1)

View all Cytochrome P450 2E1 Antibodies

SKU/Catalog Number

M00672-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband™ (monoclonal, 2C7G1) catalog # M00672-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband™ (monoclonal, 2C7G1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00672-2)

Host

Mouse

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Monoclonal

Clone Number

2C7G1

Isotype

Mouse IgG2b

Immunogen

E.coli-derived human Cytochrome P450 2E1/CYP2E1 recombinant protein (Position: H355-S493).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M00672-2 is reactive to CYP2E1 in Human, Mouse, Rat

Applications

M00672-2 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

56 kDa

Calculated molecular weight

56.849kDa

Background of Cytochrome P450 2E1

Cytochrome P450 2E1 (abbreviated CYP2E1), a member of the cytochrome P450 mixed-function oxidase system, is involved in the metabolism of xenobiotics in the body. In humans, the CYP2E1 enzyme is encoded by the CYP2E1 gene. It is mapped to 10q26.3. While it is involved in the oxidative metabolism of a small range of substrates (mostly small polar molecules), there are many important drug interactions mediated by CYP2E1. Most drugs undergo deactivation by CYP2E1, either directly or by facilitated excretion from the body. Also, many substances are bioactivated by CYP2E1 to form their active compounds. In addition, CYP2E1 is an important enzyme for the conversion of ethanol to acetaldehyde and to acetate in humans. In the conversion sequence of acetyl-CoA to glucose, CYP2E1 transforms acetone via acetol into propylene glycol and methylglyoxal, the precursors of pyruvate, acetate and lactate.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Cytochrome P450 2E1/CYP2E1 using anti-Cytochrome P450 2E1/CYP2E1 antibody (M00672-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HCCP tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cytochrome P450 2E1/CYP2E1 antigen affinity purified monoclonal antibody (Catalog # M00672-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cytochrome P450 2E1/CYP2E1 at approximately 56 kDa. The expected band size for Cytochrome P450 2E1/CYP2E1 is at 56 kDa.

Gene/Protein Information For CYP2E1 (Source: Uniprot.org, NCBI)

Alternative Names

4-nitrophenol 2-hydroxylase; CPE1; CYP2Ecytochrome P450 2E1; CYPIIE1; cytochrome P450, family 2, subfamily E, polypeptide 1; cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1; Cytochrome P450-J; EC 1.14.13.-; EC 1.14.13.n7; EC 1.14.14.1; flavoprotein-linked monooxygenase; microsomal monooxygenase; P450C2E; P450-J; xenobiotic monooxygenase CYP2E1 CPE1, CYP2E, P450-J, P450C2E cytochrome P450 family 2 subfamily E member 1 cytochrome P450 2E1|4-nitrophenol 2-hydroxylase|CYPIIE1|cytochrome P450, family 2, subfamily E, polypeptide 1|cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1|cytochrome P450-J|flavoprotein-linked monooxygenase|microsomal monooxygenase|xenobiotic monooxygenase

For more info on CYP2E1, check out the CYP2E1 Infographic

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In this infographic, you will see the following information for CYP2E1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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